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We construct gauge theories with a vector-multiplet and hypermultiplets of $(1,0)$ supersymmetry on the six-sphere. The gauge coupling on the sphere depends on the polar angle. This has a natural explanation in terms of the tensor branch of $(1,0)$ theories on the six-sphere. For the vector-multiplet we give an off-shell formulation for all supersymmetries. For hypermultiplets we give an off-shell formulation for one supersymmetry. We show that the path integral for the vector-multiplet localizes to solutions of the Hermitian-Yang-Mills equation, which is a generalization of the (anti-)self duality condition to higher dimensions. For the hypermultiplet, the path integral localizes to configurations where the field strengths of two complex scalars are related by an almost complex structure.